human escc ec109 cells  (AddexBio Inc)

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: The expression of miR-125b in ESCC tissues and cells. (A) The expression of miR-125b in ESCC and paired normal tissues was examined by qRT-PCR. (B) The expression of miR-125b in ESCC cell lines and a human esophageal epithelial cell line was examined by qRT-PCR. *P<0.05 vs. the control. ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Expressing, Quantitative RT-PCR, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: The association between the expression level of miR-125b with the clinical characteristics of ESCC patients.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Expressing

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: miR-125b inhibits ESCC cell proliferation. (A) EC109 and EC9706 cells transfected with miR-125b mimics or miR-125b inhibitors significantly increased or decreased, respectively, the expression of miR-125b compared with the control. (B) EC109 and EC9706 cells transfected with miR-125b mimics or miR-125b inhibitors both exhibited a significant decrease or increase, respectively, in the proliferation rate compared with the control. *P<0.05 vs. the control. ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Transfection, Expressing, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: miR-125b inhibits the ESCC cell cycle. EC109 and EC9706 cells transfected with miR-125b mimics significantly increased the G1 phase of the cell cycle compared with the control. *P<0.05 vs. the control. ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Transfection, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: miR-125b induces ESCC cell apoptosis. (A) EC109 and EC9706 cells transfected with miR-125b mimics significantly increased cell apoptosis compared with the control. (B) The expression of caspase-3, Bcl-2, Bax and p27 were analyzed in ESCC cell lines. *P<0.05 vs. the control. ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Transfection, Control, Expressing

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: BMF is a direct target of miR-125b in ESCC cancer cells. (A) The prediction of the binding between miR-125b and BMF as determined using TargetScan. (B) A dual-luciferase reporter assay was performed to verify the binding of miR-125b with BMF. (C) qRT-PCR assay was performed to detect the mRNA level of BMF in EC109 and EC9706 cells treated with miR-125b mimics and miR-125b inhibitors. (D) The expression of BMF was assessed in the tumor sections. *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Binding Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Expressing, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: BMF inhibits ESCC cell proliferation. (A) A qRT-PCR assay was conducted to assess the mRNA expression of BMF. (B) Western blot analysis was performed to assess the protein expression of BMF. (C) A CCK-8 assay was used to reveal the proliferation rate in ESCC cells with si-BMF transfection. (D) The cell cycle was examined in ESCC cell lines. *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, CCK-8 Assay, Transfection, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: BMF induces ESCC cell apoptosis. (A) Cell apoptosis was assayed in ESCC cell lines. (B) The protein level was assayed by western blotting in ESCC cell lines *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Western Blot, Control

Journal: Oncology Reports

Article Title: MicroRNA-125b inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by targeting BMF

doi: 10.3892/or.2018.6413

Figure Lengend Snippet: Relationship between miR-125b and BMF in ESCC. (A) The mRNA expression of BMF in ESCC tissues compared to normal tissues. (B) The mRNA expression of BMF in ESCC cell lines (EC109 and EC9706 cells) compared to an esophageal epithelial cell line (HET-1A). (C) The protein expression of BMF in ESCC tissues compared to normal tissues. (D) The protein expression of BMF in ESCC cells (EC109 and EC9706 cells) compared to an esophageal epithelial cell line (HET-1A). (E) Data analysis of relationship between the expression of miR-125b and BMF in ESCC tissues. *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.

Article Snippet: In the present study, the human ESCC cell lines (EC109 and EC9706) and human esophageal epithelial cells (HET-1A) were obtained from Riken BioResource Center (Tsukuba, Japan).

Techniques: Expressing, Control

Journal: American Journal of Translational Research

Article Title: The therapeutic response of CDDO-Me in the esophageal squamous cell carcinoma (ESCC) cells is mediated by CaMKIIα

doi:

Figure Lengend Snippet: Top 20 upregulated protein molecules modulated by CDDO-Me in Ec109 cells

Article Snippet: Human ESCC Ec109 cells was obtained from AddexBio Inc. (San Diego, CA, USA), KYSE70 and KYSE30 cells were obtained from Sigma-Aldrich Co (St Louis, MO, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: The therapeutic response of CDDO-Me in the esophageal squamous cell carcinoma (ESCC) cells is mediated by CaMKIIα

doi:

Figure Lengend Snippet: The top ten canonical signaling pathways regulated by CDDO-Me in Ec109 cells analyzed by ingenuity pathway analysis. Abbreviations: CDDO-Me, methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid; eIF2, eukaryotic initiation factor 2; p70S6K, p70S6 kinase; mTOR, mammalian target of rapamycin; RAN, ras-related nuclear protein; Nrf2, nuclear factor (erythroid-derived 2)-like 2.

Article Snippet: Human ESCC Ec109 cells was obtained from AddexBio Inc. (San Diego, CA, USA), KYSE70 and KYSE30 cells were obtained from Sigma-Aldrich Co (St Louis, MO, USA).

Techniques: Protein-Protein interactions, Derivative Assay

Journal: American Journal of Translational Research

Article Title: The therapeutic response of CDDO-Me in the esophageal squamous cell carcinoma (ESCC) cells is mediated by CaMKIIα

doi:

Figure Lengend Snippet: Representative blots of protein levels of VKORC1, CaMKIIα, NPLOC4, PSME3, and Dynamin 2 in various human ESCC cell lines and normal human esophageal epithelial cell line (Het-1A) were determined using Western blot analysis. β-Actin served as loading controls. Abbreviations: VKORC1, vitamin K epoxide reductase complex subunit 1; CaMKIIα, calcium/calmodulin-dependent protein kinase type II subunit alapha; NPLOC4, nuclear protein localization protein 4 homolog; PSME3, proteasome activator complex subunit 3; ESCC, esophageal squamous cell carcinoma.

Article Snippet: Human ESCC Ec109 cells was obtained from AddexBio Inc. (San Diego, CA, USA), KYSE70 and KYSE30 cells were obtained from Sigma-Aldrich Co (St Louis, MO, USA).

Techniques: Western Blot

Journal: American Journal of Translational Research

Article Title: The therapeutic response of CDDO-Me in the esophageal squamous cell carcinoma (ESCC) cells is mediated by CaMKIIα

doi:

Figure Lengend Snippet: Mitochondrial dysfunction signaling pathway regulated by CDDO-Me in Ec109 cells. Notes: Ec109 cells were treated with 0.5 μM CDDO-Me for 24 hours and the protein samples were subject to quantitative proteomic analysis. Red indicates an upregulation; green indicates a downregulation. The intensity of green and red molecule colors indicates the degree of down- or upregulation, respectively. Solid arrows indicate direct interaction and dashed arrows indicate indirect interaction. Abbreviations: CDDO-Me, methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid.

Article Snippet: Human ESCC Ec109 cells was obtained from AddexBio Inc. (San Diego, CA, USA), KYSE70 and KYSE30 cells were obtained from Sigma-Aldrich Co (St Louis, MO, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: The therapeutic response of CDDO-Me in the esophageal squamous cell carcinoma (ESCC) cells is mediated by CaMKIIα

doi:

Figure Lengend Snippet: mTOR signaling pathway regulated by CDDO-Me in Ec109 cells. Notes: Ec109 cells were treated with 0.5 μM CDDO-Me for 24 hours and the protein samples were subject to quantitative proteomic analysis. Red indicates an upregulation; green indicates a downregulation. The intensity of green and red molecule colors indicates the degree of down- or upregulation, respectively. Solid arrows indicate direct interaction and dashed arrows indicate indirect interaction. Abbreviation: CDDO-Me, methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid; mTOR, mammalian target of rapamycin.

Article Snippet: Human ESCC Ec109 cells was obtained from AddexBio Inc. (San Diego, CA, USA), KYSE70 and KYSE30 cells were obtained from Sigma-Aldrich Co (St Louis, MO, USA).

Techniques:

Journal: American Journal of Translational Research

Article Title: The therapeutic response of CDDO-Me in the esophageal squamous cell carcinoma (ESCC) cells is mediated by CaMKIIα

doi:

Figure Lengend Snippet: The effect of knockdown of CaMKIIα on the CDDO-Me induced apoptosis in human ESCC cells. A. Percentages of specific cell populations showed in dot plots and apoptotic cells showed in bar graphs for the silencing of CaMKIIα in Ec109 and KYSE30 cells with or without 0.5 μM CDDO-Me treatment for 24 hours. B. Representative blots of bcl-2, bax, and cleaved caspase-3 for the proteinlysate samples, which were prepared from Ec109 and KYSE30 cells treated with CaMKIIα siRNAs or 0.5 μM CDDO-Me for 24 hours. Abbreviation: CaMKIIα, calcium/calmodulin-dependent protein kinase type II subunit alapha; CDDO-Me, methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid; ESCC, esophageal squamous cell carcinoma; bcl-2, B-cell lymphoma-2; bax, bcl-2 associated X protein; siRNA , small interfering RNA.

Article Snippet: Human ESCC Ec109 cells was obtained from AddexBio Inc. (San Diego, CA, USA), KYSE70 and KYSE30 cells were obtained from Sigma-Aldrich Co (St Louis, MO, USA).

Techniques: Knockdown, Small Interfering RNA

Journal: American Journal of Translational Research

Article Title: The therapeutic response of CDDO-Me in the esophageal squamous cell carcinoma (ESCC) cells is mediated by CaMKIIα

doi:

Figure Lengend Snippet: The effect of knockdown of CaMKIIα on the CDDO-Me induced autophagy in human ESCC cells. A. Percentages of specific cell populations showed in dot plots and autophagic cells showed in bar graphs for the silencing of CaMKIIα in Ec109 and KYSE30 cells with or without 0.5 μM CDDO-Me treatment for 24 hours. B. CDDO-Me-induced autophagic death in the silencing of CaMKIIα in Ec109 and KYSE30 cellsdetermined by confocal microscopy. The level of autophagy was evaluated using a lysosome-specific fluorescence dye. The confocal microscopic images of autophagic Ec109 and KYSE 30 cells (stained in green) are also shown. C. Representative blots of beclin-1 and LC3I/II for the proteinlysate samples, which were prepared from Ec109 and KYSE30 cells treated with CaMKIIα siRNAs or 0.5 μM CDDO-Me for 24 hours. Abbreviation: CaMKIIα, calcium/calmodulin-dependent protein kinase type II subunit alapha; CDDO-Me, methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; LC 3, microtubule-associated protein 1A/1B-light chain 3.

Article Snippet: Human ESCC Ec109 cells was obtained from AddexBio Inc. (San Diego, CA, USA), KYSE70 and KYSE30 cells were obtained from Sigma-Aldrich Co (St Louis, MO, USA).

Techniques: Knockdown, Confocal Microscopy, Fluorescence, Staining, Small Interfering RNA